Queenie herself. Seductive eyes and a beautiful smile - a good potential waifu.

A level with a shard and a boss fight that takes some precise movement and Anklegator boosts to get through quickly.

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Overview

Don't waste any time at the start of the level - immediately get up and roll left to get around the plants. Keep moving quickly, if you were fast enough the falling tree trunk shouldn't stop you. Continue on through the level until you reach the second vine wall, destroy this one with a Hammer overhand and a Musket shot. The next obstacle will be another tree trunk, this one takes roll damage in addition to another overhand+musket shot combo to destroy. The big flower that seems to be blocking the way behind the trunk can be rolled next to.

You will now have reached the Brusher's Pike. Pick it up if you're in New Game mode, as at this point it has more damage output than the Cael Hammer. The way you will move on from here depends on the category, and has some possible variations depending on what happens and personal preference.

Anklegator boosts, and reaching Queen Anne

The most important mechanic that governs the Roathus Lagoon which will let you get around the place fast, is the Queen Anne boost. Every time you walk in high grass, the local Anklegator dictator will pop out, dealing damage and knocking the Kid into the air(she's only trying to help). Since we can move in mid-air by rolling, this can be heavily abused to skip walls and move over big gaps. The big thing to remember is that the boost has a 'cooldown' - it can only happen once each few seconds. In addition, the first time it will become available will be a couple seconds after reaching the area with the Brusher's Pike.

If you manage to roll right at the same time as Queen Anne hits you, you will gain a bit more distance during the boost, but this is pretty precise and dangerous, since if you do roll too early, Anne will miss you with her attack. This is not required to get enough distance to fly over the first gap.

From the Brusher's Pike platform, there are 3 gates that you have to get through to reach this level's boss.

First gate

The way you will skip this gate depends only on your game mode:

NG - Since you picked up the Pike, the boost will be available soon. Roll into the grass on your right and walk around until you get hit by Anne. As soon as you can, roll straight right. This might be slightly hard at first, but with enough distance you should make it over the gap to platform number two.

NG+ - Ignoring the pike means you have to wait longer for Anne to be able to boost you. Waiting is bad in a speedrun, so walk over to the left side of the gate. You can walk next to it on the right side of the skull, since there is no invisible wall there(it can even be done without rolling). Watch out for the ground behind, it forms quite slowly in some places.

Second Gate

There are again two ways to get through this one, but you can do either, in any category.

Method A - as you enter the platform, on the left side, a Pincushion will spawn. Kill it ASAP - in NG pike, musket shot, pike(in NG ASL, you might be able to use a grenade instead), in NG+ one musket shot up close is enough to kill it. If you did it quickly enough, the gate will start opening and you will be able to roll under it. IF you were too slow, a second Pincushion will have spawned on the right side, preventing the gate from opening. In this case you will have to kill this one as well.

Method B - take a boost from the grass in the top right, and roll around the gate in mid-air from the top side.

Third Gate

Here, you do not take the intended way through the level(top-right). Instead, you will either clip or boost around the wide gate at the bottom.

Boost - Only do this boost if you didn't take one before the second gate, since then you would have to wait too long for it. Stand in the small patch of grass over the left side of the rock wall to get it. This boost is the trickiest one because the gate's invisible wall extends really far left. To get around, aim your mid-air recovery down and a bit to the left, then, once you are below the gate, roll straight right. If you fall, don't bother trying to get another boost, go for the clip instead.

Roll Clip - This gate has a lot of holes in it that can be clipped through, but the fastest one is on its far right side, past the wall. It might take more than one roll, and requires some precise positioning(if you find this hard, you might want to go for a slightly easier but much slower clip inside the rocks on the right side; it's hard to explain, but requires changing your angle after you get inside the wall).